The authors also made this point by stating, The exact regulation of LINE-1 activity in response to BNT162b2 merits further study. (p. 1123). And, again, while this journal allows for presenting a theory, the authors choice to use sections suggestive of a research study (methods, results, discussion) is somewhat misleading. Our publication is the first in vitro study on the conversion of mRNA vaccine into DNA, inside cells of human origin. Said another way, it was an . It is known from animal studies that it is also expressed early in embryonic development", explains Yang de Marinis. Spike proteins may circulate in the body after an infection or injection with a COVID-19 vaccine. Microbiol Spectr. See this image and copyright information in PMC. As such, by definition, a modeling study cannot serve as proof. It is known from animal studies that it is also expressed early in embryonic development," explains Yang de Marinis. You should not consider the information in this site to be specific, professional medical advice for your personal health or for your family's personal health. The whole process occurred rapidly, within six hours, concluded the study, which was published by the universitys Department of Clinical Sciences. At the heart of the misconceptions about this paper is figure 1, which shows viral shedding among vaccinated (green) and unvaccinated (red) individuals. The experiment was conducted in a petri dish and aimed to answer the question of whether the mRNA in the vaccine could be converted to DNA. 1 . Its precisely what health experts and fact-checkers said for more than a year could not occur. CLAIM: A Swedish study shows that Pfizer's COVID-19 vaccine changes recipients' DNA. The study, conducted by researchers at Lund University in Sweden and published in a peer-reviewed journal, investigated the effect of Pfizers mRNA COVID-19 vaccine on a human cell line that was derived from liver cancer tissue. But keep in mind, when the mRNA or adenovirus particles do hit cells outside of the liver or the site of injection, they're still causing them to express Spike protein anchored on their surfaces, not dumping it into the circulation. shakira. doi: 10.1503/cmaj.211881. (In-vitro Swedish Study)" by Drbeen Medical Lectures WebPfizer Vaccine Becomes DNA in Liver Cells. The mRNA in the COVID-19 vaccines helps train the body to recognize a protein from the coronavirus to trigger an immune response. MR: We understood that the study would attract attention, but we think it is self-evident that this type of research should be pursued. YDM: One of the limitations of our study is that we don't know if what we observed in this cell line could also happen in cells of other tissue types, and this needs to be addressed in follow-up studies. Khehra N, Padda I, Jaferi U, Atwal H, Narain S, Parmar MS. AAPS PharmSciTech. Associate Professor According to the study, once the mRNA vaccine enters the human liver cells, it triggers the cell's DNA inside the nucleus to produce more of the LINE-1 gene expression to make mRNA. Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc. The approval came as an emergency use authorization on December 11, 2020, after clinical trials showed the vaccine was 95% effective in preventing COVID-19. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure. (in-vitro swedish study) https://lnkd.in/df6hkugy Where that paper was getting a lot of press was the idea that those pieces of DNA were then getting incorporated into the genome, and theres absolutely no evidence that that happened, Moore said. This is important for two reasons; both of which were acknowledged by the authors. First, cancerous cell lines replicate, whereas our liver cells typically are not replicating. 2023 Jan 4;45(1):400-433. doi: 10.3390/cimb45010028. yourNEWS is a hyper-local social news and advertising platform. Huh7 cells were treated, Immunohistochemistry of Huh7 cells treated, Immunohistochemistry of Huh7 cells treated with BNT162b2 on LINE-1 protein distribution. doi: 10.1056/NEJMc2107717. Andy B. on LinkedIn: Pfizer Vaccine Federal government websites often end in .gov or .mil. This means that what is seen in these cells may not be representative of what would happen in non-cancerous liver cells (or even a different line of cancerous liver cells). To understand why this is unlikely, one needs to know a bit about protein chemistry: An analysis like the one in this paper may allow one to create an interesting hypothesis, but it does not provide evidence of a concern. The Japanese regulatory agencys biodistribution study (pdf) of the Pfizer vaccine showed that some of the mRNAs moved from the injection site and through the bloodstream, and were found in various organs such as the liver, spleen, adrenal glands, and ovaries of rats 48 hours following injection. 2022 Dec 7;44(4):72. doi: 10.1007/s40656-022-00548-1. Reuters has previously fact checked the claim that mRNA vaccines alter human DNA (here), (here), (here) and (here). It is a good tool when studying molecular and cellular processes, they are easy to research, and since the cell lines are easily accessible, studies often start with various cell lines. Another aspect of protein chemistry is also important. Philadelphia, PA 19104, Know My Rights About Surprise Medical Bills. Curr Issues Mol Biol. In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage boys died in their sleep within the first week after receiving the 2nd Pfizer COVID-19 mRNA vaccine dose. Front Immunol. Lund University Childrens Hospital of Philadelphia is a charitable 501(c)(3) nonprofit organization. Well do this by highlighting a few papers that caused fear, generated confusion, or spread misinformation by looking at the interpretation, what it was based on, and why it was not correct. Imaging findings included inflammation, beading, stricturing, and dilation of the biliary tree. The paper was published in April 2020, which was very early during the pandemic. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. The messenger RNA (mRNA) from Pfizer s COVID-19 Coronaviruses, however, are not expected to do this, said Bethany Moore, chair of the University of Michigans Department of Microbiology and Immunology. 2022;44(3):1115-26. followers 129 videos. Selena Gomez. Our Standards: The Thomson Reuters Trust Principles. "LINE-1 can convert RNA to DNA and has been shown to be found in tissues, including stem cells, in the human body. Further, the newest vaccines are personalized, meaning they can target a patients Safety and Immunogenicity of Two RNA-Based COVID-19 Vaccine Candidates. Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the In the study, the authors infected a human liver cell line with the Pfizer version of the COVID-19 vaccine. DETAILS - Pfizer Vaccine Becomes DNA in The Human Liver Cells Huh7. The Centers for Disease Control and MR: The study was performed on human liver cells from one cell linecell cultures used for research purposes. 19.8M. Sudden deaths of Canadian Dr. Peter McCullough, an internist, cardiologist, and epidemiologist,wrote on Twitterthat the Swedish studys findings have enormous implications of permanent chromosomal change and long-term constitutive spike synthesis driving the pathogenesis of a whole new genre of chronic disease.. As long as the spike protein can be detected on cell-derived membrane vesicles, the immune system will be attacking the cells that release these vesicles, they said. Genomics, diabetes and endocrinologyyang [dot] de_marinis [at] med [dot] lu [dot] se, Box 117, 221 00 Lund, Sweden The study authors similarly pointed out in their Q&A that a limitation of their study is that they dont know if what we observed in this cell line could also happen in cells of other tissue types. They stated that this would need to be addressed in follow-up studies. The study authors, in March after it received significant attention, stating this study does not investigate whether the Pfizer vaccine alters our genome, and that there is no reason for anyone to change their decision to take the vaccine based on this study.. A chocolaty goodness, an amazing comforting breakfast dish that the whole family will love! We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Let's review.\r\rIf you like this content and want more, I am doing a special lifetime membership offer. (In-vitro Swedish Study) An accepted and published Swedish study demonstrates for the FILE - In this Jan. 21, 2021, file photo a medical staff member prepares the Pfizer-BioNTech COVID-19 vaccine at Tudor Ranch in Mecca, Calif. Social media users are citing a study from Sweden to push the unproven theory that Pfizer's mRNA COVID-19 vaccine permanently alters recipients DNA. Pathogenic priming likely contributes to serious and critical illness and mortality in COVID-19 via autoimmunity. The authors also pointed out that this cell line has been shown to have genetic and protein expression differences specific to RNA metabolism (p. 1123). Markus Aldn et al, Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line, Current Issues in Molecular Biology (2022). Additionally, the mRNA vaccine does in fact travel to the liver and other locations such as ovaries. The study tested whether the vaccines mRNA could be converted to DNA, and found that this was the case in certain lab-altered liver cell lines under experimental conditions. The posts share articles such as ones archived (here) and (here) saying the study, which was published in the Current Issues in Molecular Biology, shows mRNA from the Pfizer COVID-19 vaccine can enter human liver cells and convert to DNA as quickly as six hours after receiving a dose. Careers. CLAIM: A Swedish study shows that Pfizers COVID-19 vaccine changes recipients DNA. Parents PACK. Illustration of BNT162b2 was adapted from previously described literature [34]. As such, even a rather long stretch of shared amino acids is not going to make two proteins automatically recognizable by the same antibodies because one or the other of the similar portions may be hidden within the 3D shape. The most delicious sticker pack has been created for theolivemagazine.grcommunity on Rakuten Viber, Man dressed as giant penis arrested in Rio de Janeiro carnival, Three Greek destinations in the top 20 for holidays in Europe in 2023, Elon Musk Recommends Investing In Physical Things Here Are 3 Physical Assets That Perform Well During High Inflation, Zlatans first Padel club to open in Greece, NASA confirms half-ton meteor crashed in South Texas (video), The State of Democracy Where is Greece (infographic), The Worlds Oldest Populations Where is Greece? The clip comes from a longer roundtable discussion that was posted March 8 by Christian broadcasting network Daystar, during which faith leaders discussed COVID-19 vaccines with the doctors. 2022 The Childrens Hospital of Philadelphia. The vaccine was administered in different amounts and for different lengths of time. Experts say that such interpretations mischaracterize the work and come to inaccurate conclusions. The research was conducted at Lund University. The Swedish study suggests the vaccine is capable of becoming DNA, which is what the CDC claimed Getty Its precisely what health experts and fact-checkers said for more than a year could not occur. Some viruses, like HIV, are known to be able to convert RNA to DNA and then incorporate that DNA into host cells genome, which is one thing that makes the illness difficult to treat. Huh7, MeSH The researchers then investigated how different gene expressions in the cells changed over time. The genetic material delivered by mRNA vaccines never enters the nucleus of your cells, the CDC said on its web page titled Myths and Facts about COVID-19 Vaccines.. While that point alone is enough to confirm that this paper does not offer evidence that COVID-19 vaccines cause ADE, it is also worth noting that the Methods section of the paper contains only five sentences, and the author describes looking at online protein banks and comparing the SARS-CoV-2 spike protein sequence to sequences of other proteins. In the general population, the antibody response wanes over the course of time after two doses of the vaccination, but seems to be more This is 1 replication competent plasmid per 350 mRNA molecules and equates to billions of antibiotic resistant plasmids injected per person per shot . RNA is closely related to DNA, and one type, called messenger RNA or mRNA, sends instructions to the cell for different purposes. In a lab environment, i.e. Both boys were pronounced dead at home. Why liver cells and why the specific dose? Suggested accounts. Finally, as the authors pointed out, expression of LINE-1 has been shown to increase during viral infections, including with SARS-CoV-2 virus. They used different concentrations of the vaccine and multiple timepoints to measure: Some pointed to this paper as proof that COVID-19 vaccines alter DNA. Drugs and Lactation Database (LactMed) [Internet]. Pfizers BNT162b2 mRNA Covid-19 vaccine was the first vaccine against the novel coronavirus (COVID-19) approved for distribution in the United States. MR: One should consider that cell lines differ from cells in living organisms, and therefore it is important that similar investigations are also studied in humans. hasty generalization and appeal to ignorance. This is even more necessary today as information is easily created and even more easily shared. is the set of instructions to build and sustain a human being. This in itself motivates the need for further studies. Currently the Pfizer-Biontech (mRNA), Sinovac (inactivated virus) and Astrazeneca-Oxford (adenovirus-vector) COVID-19 vaccines are available for vaccination in HK. 41.2M. "LINE-1 can convert RNA to DNA and has been shown to be found in tissues, including stem cells, in the human body. And this could be seen with natural virus infection as well, Moore said. Our COVID-19 vaccine does not alter the DNA sequence of a human cell, a Pfizer spokesperson told The Epoch Times in an email. PCR primer set used to detect mRNA level and reverse-transcription of BNT162b2. 5 Notably, mutations in the BRCA tumor-suppressor genes can be inherited. The Study Has Received A Lot of Media Attention, What Are Your Thoughts on That? With this information, the author suggested that the quantity of overlap in some sequences could cause the immune system to attack ones own proteins, called autoimmunity, leading to more severe disease. Case presentation: We report a case of hepatic artery occlusion after the first dose vaccination by ChAdOx1nCov-19. YDM: About 18 percent of the vaccine accumulates in the liver just 30 minutes after the vaccine is injected in mice as reported by Pfizer in EMA assessment report, and therefore we chose to study liver cells. It is a good tool when studying molecular and cellular processes, they are easy to research, and since the cell lines are easily accessible, studies often start with various cell lines. Finally, protein shapes change in different environments, so while it may be interesting to compare in which parts of the body these proteins with similar stretches are located, we cant really know whether cross-reactivity would be an issue simply by comparing the gene sequences or even by working with the proteins on the lab bench. 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